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博士生导师张兴林教授

发布时间:2018-12-17动态浏览次数:5351


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    张兴林博士,1982年生,系浙江大学2016年“百人计划”海外高层次人才引进,欧盟(荷兰)Rubicon学者。2005年和2008年在中国农业大学分别获得学士和硕士学位,毕业后赴荷兰乌特勒之大学(Utrecht University)留学,并于2013年获得博士学位,之后在乌特勒支大学医学中心从事博士后研究;2014-2016年在英国牛津大学(University of Oxford)从事博士后研究并任职Rubicon研究员。

主要从事食源性致病菌的耐药和致病机理研究、微生物技术研发、细菌功能基因组学以及肠道菌群和益生菌研究。近年来以肠球菌、小肠炎耶尔森氏菌和布氏杆菌等为研究对象,结合新一代高通量测序技术和纳米新材料,建立和优化了M-TraMTn-seqCre-lox和热敏质粒介导的基因重组系统以及纳米颗粒磁性吸附技术等一系列国际领先的细菌微生物学方法,突破了肠球菌等重要细菌的研究瓶颈,搭建了有广泛应用价值的细菌学研究平台。在此基础上,申报人对肠球菌等细菌的耐药性、致病性、糖代谢、胆盐耐受以及遗传进化等方面进行了系统的研究,相关成果已发表在PLoS GenetJ Infect Dis Environ MicrobiolNature Nanotechnol等高影响力杂志上。

共发表SCI和国际会议论文30余篇,其中第一作者或通讯作者发表论文8,平均影响因子9.6,论文引用次数超过1200次(Google scholar 201712月)。出版英文学术书籍一本(5万余字)。主持完成欧盟(荷兰)科研项目一项(Rubicon Grant);主持浙江大学“百人计划”科研启动经费一项。参与多项欧盟重大科研项目,包括著名的微生物项目欧盟第七框架计划细菌耐药性的进化和传播EvoTAR)和欧盟第六框架计划“预防和控制多重耐药肠球菌”(ACE)等。先后十余次赴欧美参加国际学术会议并作大会报告。获得由荷兰皇家微生物协会、美国微生物协会、荷兰Eijkman-Winkler 研究所和中国国家留学基金委等颁发的多个学术奖项。

科研工作经历

 20183-至今临沂大学农林科学学院

研究员(浙江大学百人计划,正高,博导)

20166— 20183月浙江大学生物系统工程与食品科学学院

研究方向:食源性致病菌的分子流行病学以及耐药和致病机理、肠道菌群、饮食与健康的相互关系和作用机理、新一代微生物技术研发、益生菌研发和机理研究等

博士后,Rubicon研究员 (Rubicon fellow)

20149— 20166月英国牛津大学

研究方向:食源性肠道病原微生物的传播、致病机理和功能基因组学

博士后

20134— 20148月荷兰乌特勒支大学

研究方向:肠球菌的致病机理;住院病人肠道菌群的动态学研究;产细菌素益生菌研究

教育经历

博士

20089 — 20134

荷兰乌特勒支大学医学中心医学微生物系,乌特勒支大学感染与免疫中心

研究课题:肠球菌的功能基因组学、糖代谢、抗逆性、肠道内定殖力和耐药机理

硕士

 20059— 20087

中国农业大学动物医学院

研究课题:布氏杆菌的致病机理和疫苗研发

本科

20009— 20057

中国农业大学动物医学院

实习课题:禽流感病毒的流行病学研究

学术论文(IF为论文发表时的影响因子)

B. Peng†, X. Zhang,D.G.A.L. Aarts and R. Dullens. 2018. Superparamagnetic colloidal nickel nanocrystal clusters with antibacterial activity and bacteria binding ability. Nat Nano 13, pages478–482 (2018)IF38.9(†: Co-first author)

X. Zhang, V. de Maat, A.M. Guzmán, T.K. Prajsnar, J.R. Bayjanov, M. de Been, M. R.C. Rogers, M. J.M. Bonten, S. Mesnage, R. J.L. Willems, W. van Schaik. 2017. RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum. BMC Genomics 18 (1), 893IF3.7

A.M. Guzmán, J. Wijngaarden, J.C. Braat, MRC Rogers, Majoor E, E.C. Brouwer, X. Zhang,J.R. Bayjanov, M.J.M. Bonten, R.J.L. Willems, W. van Schaik. 2017. The Two-Component System ChtRS Contributes to Chlorhexidine Tolerance in Enterococcus faecium. Antimicrob Agents Chemother 61(5). pii: e02122-16.IF4.3

F.L. Paganelli,J. Huebner, K.V. Singh, X. Zhang, D. Wobser, J.C. Braat, B.E.M. Murray, J.M. Bonten, R.J.L. Willems, H.L. Leavis. 2016. Identification ofPhosphoenolpyruvate-Phosphotransferase system permease BepA involved in Enterococcus faecium endocarditis and biofilm formation by a genome-wide screening approach. J Infect Dis15;214(2):189-95.. IF6.0

A.M. Guzmán, R.T. Urbanus, X. Zhang, D. Bierschenk, A. Koekman, M. van Luit-Asbroek, J.P. Ouwerkerk, M. Pape, M. Roest, M.J.M. Bonten, R.J.L. Willems, W. van Schaik. 2015. The N-terminal domain of the thermo-regulated surface protein PrpA of Enterococcus faeciumbinds to fibrinogen, fibronectin and platelets. ‎Sci. Rep 17;5:18255. .IF5.6

R. Ladjouzi, A. Bizzini, W. van Schaik, X. Zhang, A. Rincé, A. Benachour, A. Hartke. 2015. Loss of antibiotic tolerance in Sod-deficient mutants is dependent on the energy source and arginine catabolism in enterococci. J Bacteriol DOI: 10.1128/JB.00389-15.IF2.8

X. Zhang *, M. Rogers, D. Bierschenk, M. J. Bonten, R. J. Willems, W. van Schaik. 2013. A LacI-family regulator activates maltodextrin metabolism of Enterococcus faeciumPLoS One 8(8): e72285. doi:10.1371/journal.pone.0072285. IF4.1*通讯作者)

X. Zhang *, D. Bierschenk, J. Top, I. Anastasiou, M. J. Bonten, R. J. Willems, and W. van Schaik. 2013. Functional genomic analysis of bile salt resistance in Enterococcus faeciumBMC Genomics 14:299 doi: 10.1186/1471-2164-14-299.IF4.1*通讯作者)

J. Top, F.L.Paganelli, X. Zhang, W. van Schaik, H.L. Leavis, M. van Luit-Asbroek, T. van der Poll, M. Leendertse, M. J. Bonten, R. J. Willems. 2013.The Enterococcus faecium enterococcal biofilm regulator, EbrB, regulates the esp operon and is implicated in biofilm formation and intestinal colonization. PLoS One 8 (5), e65224. IF4.1

X. Zhang, J. Top, M. de Been, D. Bierschenk, M. Rogers, M. Leendertse, M. J. Bonten, T. van der Poll,R. J. Willems, W. van Schaik. 2013. Identification of a genetic determinant in clinical Enterococcus faecium strains which contributes to intestinal colonization during antibiotic treatment. J Infect Dis 207 (11): 1780-1786. doi: 10.1093/infdis/jit076. IF6.4

F.L.Paganelli, R. J. Willems, P. Jansen, A. Hendrickx, X. Zhang, M. J. Bonten, H.L. Leavis. 2013. Enterococcus faecium biofilm formation: identification of major autolysin AtlAEfm, associated Acm surface localization, and AtlAEfm-independent extracellular DNA release. mBio doi: 10.1128/mBio.00154-13. IF5.3

X. Zhang, F. L. Paganelli, D. Bierschenk, A. Kuipers, M. J. Bonten, R. J. Willems, and W. van Schaik. 2012. Genome-wide identification of ampicillin resistance determinants in Enterococcus faeciumPLoS Genet 8:e1002804. IF9.5

F. Lebreton, W. van Schaik, M. Sanguinetti, B. Posteraro, R. Torelli, F. L. Bras, N. Verneuil,X. Zhang, A. Dhalluin, R. J. Willems, R. Leclercq and V. Cattoir. 2012. AsrR is an Oxidative stress sensing regulator modulating Enterococcus faecium opportunistic traits, antimicrobial resistance and pathogenicity. PLoS Pathog 8(8): e1002834. IF9.1

X. Zhang, J. E. Vrijenhoek, M. J. Bonten, R. J. Willems, and W. van Schaik. 2011. A genetic element present on megaplasmids allows Enterococcus faecium to use raffinose as carbon source. Environ Microbiol 13:518-28. IF5.5

X. Zhang, J. Ren, N. Li, W. Liu, and Q. Wu. 2009. Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance. Int J Biol Sci 5:570-7. (2014 IF: 4.5

Y. L. Cong, J. Pu, Q. F. Liu, S. Wang, G. Z. Zhang, X. L. Zhang, W. X. Fan, E. G. Brown, and J. H. Liu. 2007. Antigenic and genetic characterization of H9N2 swine influenza viruses in China. J Gen Virol 88:2035- 41. IF3.1

J. Liu, H. Xiao, F. Lei, Q. Zhu, K. Qin, X. W. Zhang, X. L. Zhang, D. Zhao, G. Wang, Y. Feng, J. Ma, W. Liu, J. Wang, and G. F. Gao. 2005. Highly pathogenic H5N1 influenza virus infection in migratory birds. Science 309:1206. IF31.9

其他英文学术出版物 (书籍)

Xinglin Zhang. Functional Genomics of Enterococcus faecium: Antibiotic Resistance and Niche Adaptation. (2013; book; 国际标准书号ISBN: 978-94-6108-407-1;共52819 )

获奖

国家优秀(非公派)留学生奖学金(2013

Chinese Government Award for Outstanding Self-financed Students Abroad

荷兰皇家微生物协会大会最佳报告奖(poster1st/153)(2012

Best Poster Presentation Award in Conference of Royal Netherlands Society for Microbiology

美国微生物协会学生旅费奖(2012

American Society for Microbiology Student Travel Award

Eijkman-Winkler 研究所最佳学术成果和最佳会议报告奖(2010

Eijkman-Winkler Institute Award for excellent scientific achievement and presentation

美国微生物协会学生旅费奖(2010

American Society for Microbiology Student Travel Award

中国农业大学校级优秀论文(2005

中国农业大学动物医学院优秀毕业论文(1st/96) 2005

科研项目

主持的科研项目

浙江大学百人计划项目(200 万元)2016-2022

荷兰科研组织(NWORubicon 项目(编号:825.14.010;157600.00 欧元

2014-2016

Comprehensive analyses of the essential genome and the virulence determinants of Yersinia enterocolitica

课题:小肠结肠炎耶尔森菌(食源性致病菌)的致病机理研究

参与申请或实施的重要科研项目

欧盟第六框架计划“预防和控制多重耐药肠球菌”项目

European Union Sixth Framework Programme “Approaches to Control multi-resistant Enterococci (ACE): Studies on molecular ecology, horizontal gene transfer, fitness, and prevention” under contract LSHE-CT-2007-037410.

项目由7个来自不同国家的实验室合作完成,项目总负责人Rob Willems为本人的导师。

欧盟第七框架计划细菌耐药性的进化和传播项目

该项目由来自不同国家的15 个实验室合作申请和实施,项目总负责人Rob Willems Willem van Schaik 为本人的导师。

荷兰科研组织(NWOVINI 项目

Functional genomics of Enterococcus faecium.

荷兰科研组织(NWOVIDI 项目

From harmless gut commensal to antibiotic-resistant hospital bug.

英国 Wellcome Trust项目

Bacterial immune evasion.  

国际会议报告(口头/展板)

X. Zhang, M. Rogers, et al.Combining enrichment culturing and metagenomic sequencing: characterization of populations of enterococci and Enterobacteriaceae during antibiotic therapy. 114th ASM General Conference. 114届美国微生物协会大会美国 2014

X. Zhang, D. Bierschenk, et al.Functional genomic analysis of bile salt resistance in Enterococcus faecium.7th International Conference on Gram-positive Microorganisms.第七届国际革兰氏阳性菌大会意大利 2013

X. Zhang,J. Top, et al.Identification of a genetic determinant in clinical Enterococcus faecium strains which contributes to intestinal colonization during antibiotic treatment.Gut Day. 肠道会议比利时 2013

X. Zhang,F. L. Paganelli, D et al.Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium.112th ASM General Conference112届美国微生物协会大会美国 2012

X. Zhang,F. L. Paganelli, D et al. Ampicillin resistance determinants of Enterococcus faecium.Annual EvoTAR meeting.EvoTAR年会法国 2012

X. Zhang,F. L. Paganelli, D et al.Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium.NVMM & NVvM Scientific Spring Meeting.荷兰微生物协会春季大会荷兰 2012

X. Zhang,I. Anastasiou, et al.Comprehensive identification of genes required for antibiotic resistance and bile tolerance in the nosocomial pathogen Enterococcus faecium.100th Anniversary NVvM & Scientific Spring Meeting荷兰微生物协会100周年大会荷兰 2011

X. Zhang,I. Anastasiou, et al. Development of Microarray-based Transposon Mapping in Enterococcus faecium and its application in the identification of genes required for bile resistance. ALW Platform Molecular GeneticsAnnual Meeting.ALW分子遗传学大会荷兰 2010

X. Zhang,I. Anastasiou, et al. Development of Microarray-based Transposon Mapping (M-TraM) in Enterococcus faecium and its application in the identification of genes required for bile resistance. 3rd International ASM Conference on Enterococci美国微生物协会第三届肠球菌大会美国 2010

X. Zhang,J. E. Vrijenhoek, M et al. A large conjugative plasmid confers the ability to utilize α-galactoside sugars as carbon source in E. faecium.NVMM & NVvM Scientific Spring Meeting荷兰微生物协会春季大会荷兰 2010

X. Zhang,J. E. Vrijenhoek, M et al. A novel conjugative element from Enterococcus faecium involved in α-galactoside metabolism. ESCMID Conference on Enterococci.欧洲临床微生物与传染病协会肠球菌大会西班牙 2009

X. Zhang,J. E. Vrijenhoek et al. A novel conjugative element from Enterococcus faecium involved in α-galactoside metabolism. FEMS欧洲微生物协会大会瑞典 2009

X. Zhang,J. E. Vrijenhoek, et al. Metabolism of α-galactosides in Enterococcus faecium. NVMM & NVvM Scientific Spring Meeting荷兰微生物协会春季大会荷兰 2009